【摘要】 目的 :研究涎腺鳞状细胞癌 (鳞癌 )的发生和发展规律 ,建立涎腺鳞癌动物模型。方法 :采用化学致癌剂———二甲基苯丙蒽植入颌下腺体内的方法 ,对 50只SD大白鼠颌下腺进行诱癌实验 ,观察肿瘤诱发情况。结果 :共诱发肿瘤 2 1例 ,均为鳞状细胞癌。结论 :用含有二甲基苯丙蒽的明胶海绵植入大鼠颌下腺体内能成功地建立颌下腺鳞癌的动物模型更多还原

【Abstract】 Objective:To study carcinogenesis and development of salivary gland tumor and establish an animal model of submandibular gland (SMG) tumor.Methods: Histopathological study during carcinogenesis in rat SMGs using (9,10 dimethyl 1,2 benzanthracene (DMBA) was evaluated. A total of 50 male and female Sprague Dawley (SD) rats of 8 weeks old and 180～200 g weight were obtained from the Animal Center of Henan Medical University. Under pentobarbital sodium anesthesia, the left SMGs were exposed by surgical procedure. A sponge pellet (1 0 mm×1 0 mm×1 0 mm, made by authors) was used as the carrier of the carcinogen. The sponge containing 2% DMBA (Fluka,Switzerland)/ acetone solution was implanted into the glandular tissue of the left SMGs.Four rats (2 males and 2 females) were killed after every 2 weeks of the DMBA sponge implantation. The same method of sponge implantation without DMBA was used at the right side of SMG as a control. All rats left were killed after 20 weeks. The SMGs were fixed in 10% formalin buffer solution for 24 hours, and embedded in paraffin, then 4μm thick sections were made for histopathological study. Results: The earliest tumor occurred after 4 weeks of implantation of sponge, a total of 21 lateral tumors were induced (10 females, 11 males). There was no tumor found in the controlled SMG. The peak time of tumor genesis was after 8～12 weeks of implantation, all tumors induced were squamous cell carcinomas(SCCs). The induced tumors grew slowly below the mandibles of rats, which were present as nodular masses without capsules, and the borders were not clear. They were slightly hard when palpated. The process of carcinogenesis can be described as following: squamous metaplasia of cyst like structures occurred, then SCCs were induced and invaded surrounding tissues. No metastasis was observed in regional lymph nodes and other organs. Conclusion: SCCs of SMG can be induced by implantation of DMBA. The present study supports the conclusion that all duct segments undergo squamous metaplasia, and therefore may participate in the genesis of neoplasia during experimental carcinogenesis. ;更多还原