【摘要】 目的 :研究钛种植体周结缔组织中多糖 -蛋白复合物在骨愈合过程中的变化规律及可能的作用机制。方法 :采用爱尔蓝及生物素标记的植物血凝素方法对兔下颌钛种植体周围组织中的多糖 -蛋白复合物及糖基进行观察。结果 :钛种植体周结缔组织膜、新生骨基质及成骨细胞中此物质的含量较高。结论 :多糖 -蛋白复合物在钛种植体形成骨附着及维持钛的生物相容性方面可能具有重要作用 ,并推测其与骨代谢活性密切相关。更多还原

【Abstract】 Objective: The absorption of the polysaccharide protein complex to the implant surface may be the first reaction between the implant and the surrounding tissue. So we observed the distribution and the relative amount of the polysaccharide protein complex and glycosyls in order to find out the possible functions and changing rules of these substances. Methods: 12 rabbits were selected according to the age, sex, weight, and health conditions. Bilateral mandibular premolar teeth of rabbits were extracted under abdomen anesthesia (2% glutababituratic acid), then l2 titanium implants were placed into the teeth extraction holes of one side and left the other side to be the control one. All of the implants were required to be placed 2mm below the alveolar crest, and tissue closure was achieved with mattress and interrupted single sutures. Antibiotic injections were given to the rabbits in the following 3 days. The animals were sacrificed at the end of l week, 4 weeks and 24 weeks. Tissue blocks (4mm×4mm×4mm) containing the titanium implants and the control ones were removed, then fixed with l0% formaldehyde for 2 weeks, and decalcified with 10% EDTA (pH7 0) for 8 weeks. Carefully removed the titanium implants, the tissue blocks were embedded into the paraffin wax, then the sections were obtained and stained using following methods. ①Alcian Blue Method: Alcian blue (pH2 5) staining displayed the acidic proteoglycans; Alcian blue (pH1.0) displayed the proteoglycans containing sulfonyl; Alcian blue/periodix acid shiff staining demonstrated the acidic and neutral proteoglycans respectively. Alcian blue MgCl\-2 with different ionic concentration identified the different kinds of proteoglycans. ②Lectin Method: 5 kinds of lectins were used to locate 5 kinds of glycosyls. 2 5 μg/ml ConA located the glucose; 10μg/ml PNA located the galactose; 10μg/ml WGA located the N acetylglucosamine; 25μg/ml PHA E located N acetylgalactosamine, 10μg/ml WPL loacted the fucose. Results: high level of proteoglycans and glycosyls were observed in the peri implant connective tissue during all phases of the experiment. It was also observed that polysaccharide protein complex and glycosyls reached a high level in new bone matrix as well as osteoblasts. Conclusion: Polysaccharide protein complex and glycosyls do play an impor \ \ tant role in the implant bone attachment, and titanium compatibility and may be closely related to the bone metabolic activity.更多还原