【摘要】 目的 :研究新近报道的一种与输血后肝炎有关的病毒在中国郑州地区正常人群、HBsAg阳性者、非甲至非戊型肝炎患者中的感染和基因序列变异情况。方法 :采用TTV基因组ORF1区的套式聚合酶链反应 (nested PCR)方法对血清标本进行检测 ,并对非甲至非戊型肝炎患者中的TTV分离株进行序列测定。结果 :TTVDNA在非甲至非戊型肝炎患者中检出率为 45 % (1 8/ 40 ) ;在正常体检者中检出率为 1 7.7% (1 7/ 96 ) ;在HBsAg阳性者 (ALT≤ 34IU/L)中检出率 2 2 .7% (1 0 / 44 )。TTV分离株序列与日本株 (CLON2 2 )相对应位置的核苷酸同源性为 99.1 %。结论 :TTV在非甲至非戊型肝炎患者TTV的感染率明显高于其他人群 ,可能是非甲至非戊型肝炎的主要致病因子。更多还原

【Abstract】 Aim:To investigate the prevalence of TTV infection in different population. Methods:DNA of TTV was tested by polymerase chain reaction with nested primers in serum of all peoples. The partial gene of TTV isolated from the patients was cloned and sequenced. Results: DNA of TTV was 45% (18/40) positive for patients with non A～E hepatitis, 17.7% (17/96) positive for the controls,22.7% (10/44) positive for the people with HBsAg positive(ALT≤34 IU/L). The partial sequence of TTV isolates showed 99.1% nucleotide identity over the corresponding region of TTV in Japan (CLON22). Conclusion: TTV would be the major cause of patients with non A～E hepatitis.更多还原