【摘要】 目的 :观察蝎毒抗癌多肽 (antineopasticpolypeptidefromButhusmartensiivenom ,APBMV)对小鼠肝癌 (hep atoma 2 2 ,H2 2 )的生长抑制作用及带瘤小鼠免疫功能的影响。方法 :①将接种H2 2 细胞 2 4h后的带瘤小鼠 45只随机分为非正常对照组、5 Fu组、APBMV针剂组和胶囊组 4组 ,给予不同的药物处理 1 0d后 ,观察瘤块及脾脏重量的变化 ,计算抑瘤率和脾重指数 ;②接种H2 2 细胞 2 4h后的带瘤小鼠 40只随机分为非正常对照组、5 Fu组、APBMV针剂组和APBMV针剂 +5 Fu组 ,给予APBMV针剂和 /或 5 Fu 5d后 ,分别观察带瘤小鼠NK细胞活性及淋巴细胞转化率。结果 :① 5 Fu和APBMV针剂组对H2 2 的抑瘤率分别达 47.81 %和 40 .0 7% ,与非正常对照组相比差异均有显著性 (P <0 .0 5 )。APBMV针剂及胶囊组的脾重指数分别为 70 .0 2mg/1 0g和 71 .2 2mg/1 0g,与非正常对照组相比P <0 .0 1 ;②APBMV针剂组及APBMV针剂 +5 Fu组带瘤小鼠的NK细胞活性明显增强 ,淋巴细胞转化指数明显增高(P <0 .0 5 )。结论 :APBMV针剂可抑制H2 2 的生长 ,并增强带瘤小鼠机体的免疫功能更多还原

【Abstract】 Aim: To observe the influence of antineoplatic polypeptide from Buthus martensii venom (APBMV) on the growth of hepatoma 22(H 22 )and the immunity of H 22 bearing mice. Methods: ①The 45 mice inoculated by H 22 cells for 24 hours were divided into 4 groups:non normal control group,5 Fu treated group,APBMV injection and capsule groups. H 22 bearing mice were treated by different chemicals for 10 days and the weight changes of H 22 tumor and spleen were observed. The tumor inhibitory rate and spleen weight index were calculated. ②The 40 mice inoculated by H 22 cells for 24 hours were divided into 4 groups:non normal control group,5 Fu treated group,APBMV injection or/and 5 Fu groups. H 22 bearing mice were treated with APBMV injection or/and 5 Fu for 5 days. The activity of NK cell and the transformation rate of lymphocytes were observed. Results: ①Inhibitory rates in 5 Fu and APBMV injection groups were 47.81% and 40.07%, respectively. Compared with the non normal control group, the differences were significant ( P <1.05). The spleen weight indexes were 70.02 mg/10 g and 71.22 mg/10 g in APBMV injection and capsule groups, respectively. Compared with the non normal control group, the differences were significant ( P <0.01). ②The activity of NK cell was enhanced remarkably and the transforming rate of lymphocytes was increased evidently in both APBMV injection group and APBMV injection+5 Fu group ( P <0.05). Conclusion: APBMV could inhibit the growth of H 22 and strengthen the immunity function of H 22 brearing mice.更多还原