【摘要】 目的 :了解多环芳烃接触者遗传指标和血清癌基因蛋白P2 1表达水平及其在肺癌早期诊断中的意义 ,为多环芳烃作业者的劳动保护提供科学依据。方法 :对接触多环芳烃化合物的焦炉工 2 4名、沥青工 2 7名和健康对照组 31名的染色体畸变率和血清癌基因蛋白P2 1水平进行了检测。结果 :染色体异常检出率 ,焦炉工和沥青工均显著高于对照组 (P <0 .0 0 5 ) ;染色体裂隙次数 ,焦炉工和沥青工均显著高于对照组 (P <0 .0 1) ;染色体结构、数目畸变数 ,焦炉工显著高于对照组 (P <0 .0 1)。血清P2 1水平 ,焦炉工、沥青工均显著高于对照组 (P <0 .0 5 ) ,沥青工显著高于焦炉工 (P <0 .0 5 )。结论 :多环芳烃化合物具有遗传毒性 ,并可致血清P2 1蛋白水平升高。更多还原

【Abstract】 Aim: The carcinogenesis of polycyclic aromatic hydrocarbon (PAHs) has been proved by epidemiology and animal experiment. Free radical induced by polycyclic aromatic hydrocarbon(PAHs) could cause bionmembrane damage, decreasing of immunization function, chromatosome aberration and then cause tumor. With the development of tumor molecular biology, the correlation between chromatosome aberration and expression of tumor gene was investigated. Ras activation occurs at early stage of carcinogenesis and its expression (P21 protein) may be an useful index distinguishing high risk individual of tumor. To understand early diagnostic significance of chromatosome aberration and P21 for lung tumor and to explore early monitoring index of lung tumor and supply scientific evidence of labor protection for workers exposed to PAHs. Method: Method of epidemiology was used to study chromosome aberration and P21 in coke oven workers (24 cases), pitch workers (27 cases) and health workers (31 cases). Result: Abnormal rate of chromosome was higher significantly in coke oven worker group (89.5%) and pitch worker (87.5%) than in control group (33.7%) ( P <0.05). There was no difference between coke oven worker group and pitch worker group. Frequency of chromosome crack were higher obviously in coke oven worker group (33/1 900 cells) and pitch worker group ( 45/2 400 cells) than in control group (3/1 200 cells) ( P <0.01). The relative area of Western blotting representing P21 in serum, the level of P21 in serum was higher markedly in coke oven worker group (2 702±1 532) and pitch worker group (4 398±1 768) than in control group (1 200±757) ( P <0.05), higher in pitch workers group than in coke oven worker ( P < 0.05 ). Conclusion: Polycyclic aromatic hydrocarbon (PAHs) had genetic toxicity; ras activation resulted in increased expression P21 protein. The quantitative analysis of P21 in serum was a good marker and positive sample should be monitored and further studied.更多还原