【摘要】 目的 :探讨互隔交链孢霉诱导食管癌的发生机制。方法 :用交链孢酚 (AOH)体外处理不同时间 (2 4h ,1周和 3周 )胎儿食管上皮组织 ,提取该组织的DNA ,从 10例食管癌组织及 10例癌旁组织中分别提取DNA ,设正常胎儿食管上皮DNA为对照。EcoRI酶切 ,Southern转移 ,与EGFr癌基因 ,Rb抗癌基因探针杂交。结果 :正常对照组中EGFr、Rb无扩增及丢失 ;AOH诱导 2 4h的胎儿食管上皮中即有EGFr癌基因的扩增 ,并一直持续到第 3周 ;而AOH诱导 2 4h ,1周和 3周的胎儿食管上皮未发现抗癌基因Rb的丢失 ;食管癌组织中有 2例EGFr扩增 (2 /10 ) ,2例Rb完全缺失和部分丢失 (2 /10 ) ,癌旁组织中有 4例EGFr扩增 (4/10 ) ,Rb无任何缺失 (0 /10 )。结论 :AOH激活了EGFr癌基因 ,EGFr癌基因的激活可能是食管上皮癌变的启动性变化 ,Rb抗癌基因的丢失在AOH诱导的食管癌发生中可能是一种较晚期的改变。更多还原

【Abstract】 Aim: To study the mechanism of esophageal cancer induced by alternariol (AOH). Methods: DNA was extracted from the human fetal esophagus (HFE) treated with AOH for 24 hours,1 week and 3 weeks in vitro. As controls, DNA from normal HFE, and DNA from esophageal carcinoma tissues, “normal” epithelium tissue near carcinoma region were extracted respectively. DNA samples were digested with EcoRI enzyme and subjected to Southern blotting ,and hybridized with EGFr and Rb genes probed . Results: There was amplification of EGFr gene in HFE induced by AOH in 24 hours, and the amplification continues to the third week.There were not any deletion of Rb gene in HFE treated with AOH for 24 hours in the first week and the third week. Among 10 esophageal cancer tissues, there were amplification of EGFr gene in 2 cases,and complete or partial deletion of Rb gene in 2 cases and in 10 “normal” epithelium tissues near carcinoma region were found amplification of EGFr gene in 4 cases,no deletion of Rb gene. Conclusion: AOH could activate EGFr. The activation of EGFr gene is probably the initiated change in the canceration course of esophageal epithelium, and the inactivation of Rb anti oncogene might be involved as late event in esophageal carcinogenesis.更多还原