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cAMP类似物对人恶性胶质瘤细胞BT325增殖与分化的影响

Effects of cAMP analogues on proliferation and differentiation of human glioblastoma BT325 cells

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【作者】 陈奎生尹先印殷智榕

【Author】 Chen Kuisheng; Yin Xianyin;Yin zhi rong (Department of Pathology, Henan Medical Universyty, Zhenzhou 450052 (Department of Neurosurgery,the Second Affiliated Hospital Henan Medical University, Zhengzhou 450003)

【机构】 河南医科大学病理教研室!郑州450052河南医科大学二附院神经外科!郑州450003

【摘要】 目的:观察cAMP类似物8-Br-cAMP对人恶性胶质细胞HT325增殖与分化的影响。方法:在人恶性胶质瘤细胞BT325中加终浓度为10-5mol/L的8-Br-cAMP体外培育24h后,应用酸解DNA及甲绿-派洛宁染色技术在同一标本上鉴别增殖型细胞和分化型细胞。结果:8-Br-cAMP处理组增殖型细胞占67%,分化型细胞占33%;而对照组增殖型细胞占85%,分化型细胞占15%。结论:8-Br-cAMP对BT325细胞有抑制增殖和促进分化效应。

【Abstract】 To observe the effects of cAMP analogs 8 - Br - cAMP on proliferation and differentiation of human glioblastoma BT325 cells. Methods: After the BT325 cells were cultured at final concentration of 10- 5 mol/L 8 - Br - cAMP for 24h, both proliferation and differentiation Pattern cells were identified on the same slide by using HCl hydrdysis of DNA and metlcyl green - pyronin stain technique . Results: The proliferation pattern cells decreased, while the differentiation pattern cells increased . Conclusion : The results suggest that 8 - Br - cAMP could facilitate BT325 cell differentiation and suppress BT325 cell proliferation.

【关键词】 8-Br-CAMP细胞增殖细胞分化BT325细胞
【Key words】 8-Br-cAMPcelldifferentiationproliferationBT325cells
  • 【文献出处】 河南实用神经疾病杂志 ,HENAN JOURNAL OF PRACTICAL NERVONS DISEASES , 编辑部邮箱 ,2000年01期
  • 【分类号】R730.264
  • 【被引频次】1
  • 【下载频次】24
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