【摘要】 根据已发表的牛传染性鼻气管炎病毒 (IBRV )gB基因序列 ,应用 0ligo4.1程序设计一对引物PB1/PB2 ,分别对IBRVLA株、洛精、美精、BarthaNu/ 67株、B7株、云南 -1和云南 -2分离株进行了PCR扩增。结果显示 7株IBR病毒DNA均有 3 3 9bp的特异性片段。用此引物对其同属的伪狂犬病毒 (PRV)、马立克氏病毒 (MDV)、鸡传染性喉气管炎病毒 (ILTV)进行扩增 ,均未见特异性条带 ,证明该引物是特异的 ;检测PCR的敏感性表明 ,该法可检测出 3 pg的病毒DNA。更多还原

【Abstract】 One pairs of primers PB1/PB2 were designed using oligo 4.1 program based on the published sequences of gB genes of IBRV.The DNA sextracted from the IBRV strains LA,Luojing,Meijing,Bartha Nu/67,B7,Yunnan-1 and Yunnan-2 were subjected to amplifying by PCR using the primers.The amplification yielded a specific band of 339 bp for all the IBRVs when using PB1/PB2.There were no specific amplifications for pseudorabies(PRV),Marek’ disease virus(MDV) and infectious laryngotracheitis virus(ILTV).The PCR was shown to be sensitive and could detect as low as 3 pgs of viral DNAs.更多还原