【摘要】 ［目的］ 为监测外环境中是否还有脊髓灰质炎 （Ｐｏｌｉｏ） 野病毒株循环。［方法］ 在曾是Ｐｏｌｉｏ 流行区的地区， 于１９９７～１９９８年定点采集０～４岁儿童粪便５３８ 人份， 河塘水８８份， 市自来水厂源水８份和出厂水６份。［结果］ 从粪便和水中分离到ＰＶⅠ型２８株、ＰＶⅡ型１株、ＰＶⅢ型５ 株、ＮＰＥＶ４２株。分离的ＰＶ温度特征试验， 判为弱毒（疫苗） 株； ＲＴ－ＰＣＲ型内分析亦为疫苗（Ｓａｂｉｎ） 相关株。［结论］ 在具有对Ｐｏｌｉｏ高免疫屏障的人群和外环境中， 仍有Ｐｏｌｉｏ 病毒循环， 但均为疫苗相关株。更多还原

【Abstract】 Objective]To find out the circulation of wild strains of poliovirus [Methods]538 fecal specimens were sampled from 0~4 year old healthy children,88 samples from river and pool water,8 samples from source water and 6 treated water samples of water plants were sampled and analyzed in areas which were polio epidemic area before during 1997~1998 [Results]28 strains of type Ⅰ poliovirus,1 strain of type Ⅱ poliovirus,5 strains of type Ⅲ poliovirus and 42 strains of NE poliovirus were isolated from fecal specimens and water samples,which were identified to be vaccine related strains(low toxicity) by sensitive test of temperature and RT PCR intratypic analysis [Conclusion]The circulation of vaccine related strains of poliovirus still existed among population with high immune barrier to poliovirus and in environment更多还原