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外环境中脊髓灰质炎病毒监测研究

Monitoring on Poliovirus in Environment

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【作者】 赵文彬周克捷李家富林红玉卞永红

【Author】 ZHAO Wen bin,ZHOU Ke jie,LI Jia fu,et al Lianyungang Sanitation and Anti epidemic Station,222003

【机构】 江苏省连云港市卫生防疫站!222003

【摘要】 [目的] 为监测外环境中是否还有脊髓灰质炎 (Polio) 野病毒株循环。[方法] 在曾是Polio 流行区的地区, 于1997~1998年定点采集0~4岁儿童粪便538 人份, 河塘水88份, 市自来水厂源水8份和出厂水6份。[结果] 从粪便和水中分离到PVⅠ型28株、PVⅡ型1株、PVⅢ型5 株、NPEV42株。分离的PV温度特征试验, 判为弱毒(疫苗) 株; RT-PCR型内分析亦为疫苗(Sabin) 相关株。[结论] 在具有对Polio高免疫屏障的人群和外环境中, 仍有Polio 病毒循环, 但均为疫苗相关株。

【Abstract】 Objective]To find out the circulation of wild strains of poliovirus [Methods]538 fecal specimens were sampled from 0~4 year old healthy children,88 samples from river and pool water,8 samples from source water and 6 treated water samples of water plants were sampled and analyzed in areas which were polio epidemic area before during 1997~1998 [Results]28 strains of type Ⅰ poliovirus,1 strain of type Ⅱ poliovirus,5 strains of type Ⅲ poliovirus and 42 strains of NE poliovirus were isolated from fecal specimens and water samples,which were identified to be vaccine related strains(low toxicity) by sensitive test of temperature and RT PCR intratypic analysis [Conclusion]The circulation of vaccine related strains of poliovirus still existed among population with high immune barrier to poliovirus and in environment

【基金】 连云港市科学基金!资助项目(SH97008)
  • 【文献出处】 环境与健康杂志 ,JOURNAL OF ENVIRONMENT AND HEALTH , 编辑部邮箱 ,2000年01期
  • 【分类号】R512.4
  • 【被引频次】5
  • 【下载频次】52
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