【摘要】 应用 Enviro Amp PCR-反向杂交法 (涉及 5S r RNA基因和 mip基因 )和半巢式 PCR(涉及 1 6S r RNA基因 )成功检测人工气溶胶化的军团菌 ,这些方法进一步用于检测空调系统冷却塔的水及由其产生的气溶胶中的军团菌 ,从空气样品中检测到军团菌属及嗜肺军团菌 .检测的冷却塔水样品 1 0 0 %含军团菌属细菌 ,其中 41 .6%含嗜肺军团菌 .2种方法相比 ,半巢式 PCR法更经济、方便 .更多还原

【Abstract】 Artificial aerosolized Legionella was collected into buffer and detected by using EnviroAmp Legionella detection kit (5S rRNA and mip genes) method and seminested PCR (16S rRNA gene) method.These methods were also used to detect Legionella in aerosol produced from operating cooling towers which were contaminated by Legionella. From 200L of collected air sample,not only Legionella sp.but also L.pneumophila could be detected.Both methods had similar sensitivity.Seminested PCR method,however,was more simple and more economical,although it failed to detect the species L.Pneumophila because of only genus specific primers being used in this study.The results also indicated that 100% of cooling tower water samples tested contained Legionella sp.and 41.6% of them contained L.pneumophila.更多还原