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SDSS-D2EHPA/异辛烷混合反胶团体系提取细胞色素C的研究
Protein Extraction by SDSS-D2EHPA/Isooctane Mixed Reverse Micelles System
【摘要】 用 SDSS- D2 EHPA/异辛烷混合反胶团体系提取蛋白质细胞色素 C。研究了提取时间、温度、相体积比以及助提取剂用量对提取率的影响。结果表明 ,提取率接近 1 0 0 % ,并且提取时间短 ,为 5min;提取可在室温下进行
【Abstract】 Studies are made on the extraction of cytochrome C by SDSS D 2EHPA/isooctane mixed reverse micelles system. The effect of temperature, time and phase volume ratio on the extraction yield of cytochrome C is discussed. The results show that the capacity of SDSS D 2EHPA/isooctane mixed reverse micelles to extract cytochrome C is so high that nearly 100% of the extraction yield can be obtained. The extraction time is as short as 5 min, and the extraction can be carried out at room temperature.
【基金】 国家自然科学基金重点资助项目! ( 2 96730 0 9)
- 【文献出处】 淮海工学院学报(自然科学版) ,JOURNAL OF HUAIHAI INSTITUTE OF TECHNOLOGY , 编辑部邮箱 ,2000年03期
- 【分类号】O629
- 【被引频次】2
- 【下载频次】98