【摘要】 目的　观察干扰素 (IFN a)和肿瘤坏死因子 (TNF a)单独应用或与化疗药物联合作用于肿瘤细胞的凋亡诱导作用。方法　形态学观察和流式细胞仪检测细胞因子对培养的人早幼粒白血病细胞(HL 6 0 )、小鼠淋巴细胞性白血病细胞 (L 12 10 )和小鼠肝癌细胞 (H2 2 )的凋亡诱导作用。结果　用光学显微镜、荧光显微镜和电子显微镜均观察到细胞因子诱导肿瘤细胞凋亡的典型形态学改变 ,流式细胞仪检测结果表明 ,干扰素与阿糖胞苷 (Ara c)合用或肿瘤坏死因子与阿糖胞苷合用可以显著提高肿瘤细胞凋亡率 ,并可明显抑制肿瘤细胞DNA的合成 ,使S期和G2 M期的细胞显著减少 ,而G0 G1 期细胞增多 ,因而使增殖指数 (PI)显著降低 (P <0 .0 1)。结论　某些细胞因子与化疗药联合使用可以起到增强抗肿瘤的疗效作用。更多还原

【Abstract】 Objective To observe the inducing effect of cytokines on apoptosis of tumor cells when interfe ron a(IFN a) and tumor necrosis factor a(TNF a) were applied alone or together with chemotherapeutical drugs.Methods The inducing effects of cytokines on apoptosis of tumor cells(HL60,L 1210 and H22) in vitro were observed by means of optical microscope,fluoroscence microscope,electron microscope and flow cytometry. Results The typical characteristics of apoptosis were found and the result of flow cytometry showed that Interferon a and Tumor Necrosis Factor a could increase considerably the apoptosis rate(P<0.01)and synthesis of DNA of tumor cells were inhibited obviously,the cell numbers in the S and G 2M phase were decreased,whereas the cells in the G 0/G 1 phase were increased, so the proliferation index(PI) were reduced.Conclusion Interferon aand Tumor Necrosis Factor a combined with chemotherapeuticdrugs could increase antitumor effect.更多还原