【摘要】 以玉米黄化苗为生物材料 ,将其捣碎后 ,通过差速离心获得线粒体 ,使用超声波将其破碎 ,用 2 %Triton X-1 0 0溶膜 ,超速离心 ,硫酸铵沉淀 ,DEAE-C32 层析纯化琥珀酸脱氢酶 ,纯化倍数为 1 2倍 .电泳图谱显示一条带 .更多还原

【Abstract】 Succinate dehydrogenase is partially purified from zea etiolated seedling tissue by differential centrifugation,sonic oscillation,solubilization of the enzyme from the submitochondrial particles with Triton X 100,ammonium sulfate fractionation and DEAE C 32 chromatography.Through PAGE,one major band is detected on gel. [WT5HZ]更多还原