【摘要】 以美国红葡萄、黑葡萄为供试材料 ,对葡萄试管苗快繁过程中 ,从取材、材料消毒、诱导茎芽萌发、增殖、生根到移栽这几个关键环节进行了研究。试验表明 ,在葡萄旺盛生长的 6,7,8月份取材 ,以侧芽做外植体比顶芽易存活 ;材料用 75%酒精处理 2 0s ,再用 0 1%升汞浸泡 4min ,存活率可达 70 %～ 85%。 6-BA浓度为 0 5mg/L时诱导芽增殖萌发效果最佳 ,每 4周可增殖 3～ 4倍。在生根培养基中NAA浓度为 0 2mg/L时 ,生根率达 90 %左右。分步移栽 ,可使移栽成活率达 80 %。更多还原

【Abstract】 Experiments were conducted to study various factors affecting rapid propagation of grape (America red grape and America black grape) in vitro.Selection and sterilization of plant material,induction of shoot bud and multiple shoots,shoot rooting and plant transplanting were all studied. The original shoots from grape in June,July and August showed good potential for culture.The survival rate of axillary buds was higher than that of the apical buds.By dipping the original shoots in 75% ethanol for 20 seconds, and then treating with 0 1% mercuric chloride for 4 minutes, the survival rate of shoot buds was up to 70%-80%.The 0 5mg/L 6-BA solution significantly stimulated budding and shoots elongation,the propagation coefficient was 3-4 times per week.Shoot rooting rate reached 90% when shoots were planted in the root induction medium NAA (0 2mg/L).The survival rate of transplanted plants was around 80%,when they were conducted step by step.更多还原