【摘要】 以普通小麦细胞质雄性不育保持系 75 3369B为材料 ,分别用限制性内切酶EcoRⅠ和MboⅠ对 75 3369B的mtDNA进行完全酶切和部分酶切 ,构建了两个基因文库。其中用EcoRⅠ酶切得到的库 (以下简称ME库 )由 350个重组子构成 ;MboⅠ酶切所构建的库 (以下简称MM库 )由 4 50个重组子构成 ,MM库的库容量是 98 12 %。同时 ,讨论了两种建库方法的优缺点更多还原

【Abstract】 Cytoplasmic male sterility(CMS)line 75-3369A’s maintainer 75-3369B of common wheat was studied.mtDNA was digested completely with EcoRⅠ and partly with MboⅠ and two gene libraries(ME and MM)of mitochondrial DNA(mtDNA)of 75-3369B was constructed.ME consisted of 350 clones and MM contained 450 clones.MM library included 98 12 percent of the mitochondrial genome.The advantage and disadvantage of the two methods of gene library construction were also discussed.更多还原