【摘要】 利用苯乙酸与捕捉剂反应生成的沉淀 ,确定固定化青霉素G酰化酶的催化活性部位 .对不同酶活的固定化青霉素G酰化酶进行了活性定位的研究 ,发现酶活的部位集中分布在载体的表面上 ,且分布不均匀 ,而断面上几乎没有酶活存在 ,证明了该方法的可靠性与准确性更多还原

【Abstract】 The active site of immobilized penicillin G acylase can be determined according to the precipitant produced in the reaction between phenylacetic acid and capture reagent. Moreover, the localization of the immobilized penicillin G with different activity is done as well. It is found that most enzyme distributes on the surface of carrier, but few of enzymes distribute in the interior of the carrier, and distribution of enzyme is not uniform on the surface of carrier. The present method is applicable and accurate.更多还原