【摘要】 为了能对固定化青霉素G酰化酶进行X射线微区分析 ,筛选了能捕捉酶活的合适的底物与捕捉剂的体系 ,青霉素G钠作为底物 ,FeCl3 作为捕捉剂 ,底物经固定化青霉素G酰化酶水解产生苯乙酸 ,后者与捕捉剂反应生成沉淀 ,可以确定固定化青霉素G酰化酶的催化活性部位 ;还对捕捉剂与底物、固定化青霉素G酰化酶与载体以及载体与底物之间的相互作用进行了研究 ,找到了可用于对固定化青霉素G酰化酶活性进行X射线微区定位的捕捉剂.更多还原

【Abstract】 The experiment is performed for the X-ray microanalysis of immobilized penicillin G acylase. In the experiment, the appropriate systems of substrate and capture reagent, which can capture active site of enzyme, are carefully selected. Benzylpenicillin sodium and ferric chloride act as substrate and capture reagent respectively. Pheyactic acid is reactive when the substrate is hydrolyzed by immobilized penicillin acylase. The active site of immobilized penicillin G acylase can be determined according to the precipitant produced in the reaction between phenylacetic acid and capture reagent. Further more, the interaction between the capture reagent and substrate, between immobilized penicillin G acylase and carrier, and between carrier and substrate are studied as well .The capture reagent which can locate the active site of immobilized penicillin G acylase by X-ray microanalysis is found.更多还原