【摘要】 目的了解贵州地区庚型肝炎病毒感染状况，分析度型肝炎病毒贵州株的基因特点。方法以废型肝炎病毒（ＨＧＶ）的ＮＳ_３区两对寡核苷酸为引物，用逆转录套式聚合酶链反应（ＲＴ－ｎＰＣＲ）检测不同人群血清中庚型肝炎病毒核酸(ＨＧＶ ＲＮＡ，并用ＰＣＲ产物直接测序分析３价阳性血清５’非编码区的部分核苷酸序列。结果６０位正常人，３７例血液病，８６例肝病患者及１０７例静脉药瘾者血清ＨＧＶ ＲＮＡ阳性率分别为１．７％（１／６０），１６．２２％（６／３７），２５．５８％（２２／８６）和３５．５１％（３８／１０７）。在肝病组中，急性非甲～戊型肝炎，慢性肝炎，肝硬化的ＨＧＶ ＲＮＡ阳性率分别为２５．０％（３／１２），２３．５３％（４／１７）和２６．３２％（１５／５７）。测定的１５６个核苷酸中，３株贵州株的同源性均高于９４．２％。代表株Ｇ_３与国外株（Ｕ３６３８０，Ｕ４４４０２， Ｕ４５９６６）同源性分别为８３．９％， ９０．４％和８９．１％。与国内株（Ｃ９６４， Ｇ００６）同源性分别为９２．７％和９６．８％。３株贵州林均存在缺失突变。结论贵州存在ＨＧＶ感染，静脉药瘾者中有较高的ＨＧＶ感染率。ＨＧＶ可能是急性非甲～成型肝炎的病原因子。３株贵州株?更多还原

【Abstract】 Objective To investigate the prevalence of hepatitis G virus infection and analysis partial nucleoti- de sequence. Methods Two hundred and ninety sera were collected from different population in Guizhou area and HGV RNA in the sera was detected by reverse transcription nested polymerase chain reaction (RT-nPCR). The 156bp fragments from 5’NCR of HGV isolaed from the sera of three patients with non-A-E hepatitis were purified and directly sequenced. The nucleotide sequence of a representative strain (HGV-G3) was compared with those of the HGV U36380, U44402, U45966 reported in American and HGV C964, G006 reported in China. Results The positive rates of HGV RNA in 60 normal adults, 107 intravenous (IVDUS), 37 patients with homeopathy, 86 pa tients with liver diseases were 1.7% (l/60), 35.51% (38/lo7), 16.22% (6/37) and 25.58% (22/86),respec- tively. The nucleotide homology of the 5,NCR was from 94.2% to 97.4% within 3 HGV Guizhou strains (G1- G3). AS compared with published HGV sequence, the nucleotide homology between HGV-G3 and HGV U3638, U44402, U45966, HGV C964, HGV-G006 were 83.9%, 90.4%, 89. l%, 92.9% and 96.8%, respectively. The deletion of nucleotides was distributed in sequence. Conclusion There are hepatitis G virus infection in Guizhou area, the infection rate was higher in IVDUs. The nucleotide homology was higher between strains from China than that between strains from Guizhou and abroad.更多还原