【摘要】 利用单克隆抗体 ELISA,研究了 UV- B对水稻 DNA中 CPDs和 6- 4PPs的诱导形成 ,并证实了水稻幼苗中 DNA损伤的光修复和暗修复的存在 .经 UV- B处理的水稻幼苗 ,DNA中 CPDs和 6- 4PPs的积累随 UV- B处理时间的增加而上升 .水稻幼苗 DNA损伤的暗修复能力较低 ,2 4 h内仅修复约 2 0～ 30 % .与暗修复相比 ,DNA损伤的光修复进程较快 ;CPDs的光修复在 2 0 min内可修复 50 % ;而 6- 4PPs的光修复需要 1 h才能完成 50 %修复 .以上结果表明 ,UV- B诱导的水稻 DNA损伤的修复以光修复为主 ,且 CPDs和 6- 4PPs的修复活性与其它植物存在差异更多还原

【Abstract】 By using monoclonal antibody enzyme linked immunosorbent assay (ELISA),induction and repair of damage to DNA in rice seedlings irradiated with UV B were studied in the present paper.UV B induced DNA damage,namely,cyclobutane pyrimidine dimers (CPDs) and (6 4) photoproducts (6 4PPs) in rice seedlings were found to be accumulated with increasing time of UV B treatment.The dark repair of both types of photolesions was confirmed in rice seedlings.Dark repair was less efficient as compared with photorepair,only about (20～30)% of initial damage was removed within 24h.Photorepair of CPDs was very fast,with 50% removal within 20min.By contrast,50% of initial 6 4PPs were removed in 1h.These results indicate that photorepair is the predominant mode of DNA repair in rice seedlings,and repair activities in rice seedlings are different from those in Arabidopsis,in which 6 4PPs are photorepaired more rapidly than CPDs.更多还原