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微波对体外培养视网膜神经细胞损伤的光镜与电镜观察

Light and Electron Microscopic Observation on the Damage of Retinal Nervous Cells Cultured in vitro Induced by Microwave

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【作者】 刘学东龚书明杨瑞华陈景元刘秀红邓中荣

【Author】 Liu Xuedong et al (Division of Hygiene,The 4 th Military Medical University,Xian 710032 China)

【机构】 第四军医大学军队卫生学教研室!西安市710032

【摘要】 为了探讨 2 45 0MHz微波对眼底的损伤 ,采用体外培养视网膜神经细胞 ,按微波强度分为 3组进行微波辐照 1h ,进行光镜和透射电镜观察。结果表明 ,30mW /cm2 微波辐照可使视网膜神经细胞发生凋亡 ,6 0mW /cm2 微波辐照可导致细胞坏死。可见 ,微波可损伤体外培养的视网膜神经细胞 ,诱发细胞凋亡 ,随着微波辐照强度的增大 ,损伤程度加重。

【Abstract】 To study the damage of ocular fundus induced by microwave,retinal nervous cells were cultured in vitro and divided into 3 groups according to microwave intensity.The cell morphology was observed by light and electron microscopes after exposed to microwave for 1 hour.The results showed that the retinal nervous cells underwent apoptosis after exposed to 30 mW/cm 2 microwave,some portion of cells showed necrotic after exposed to 60 mW/cm 2 microwave.It suggested that the retinal nervous cells could be damaged and underwent apoptosis due to microwave exposure,and the damage was consistent with the microwave intensity.

  • 【文献出处】 工业卫生与职业病 ,INDUSTRIAL HEALTH AND OCCUPATIONAL DISEASES , 编辑部邮箱 ,2000年03期
  • 【分类号】R135.1
  • 【被引频次】6
  • 【下载频次】71
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