【摘要】 从上海市郊农村草堆的土壤中分离到一株分解纤维素的菌 ,经初步鉴定为绿色木霉。该菌最适生长温度为 2 8℃。在本实验条件下 ,该菌的产酶高峰在 96h左右 ;对木薯渣的水解能力最强 ,滤纸的水解活力 (FilterPaperActivity ,FPA)为 1 0 .8u/ml发酵液。其酶的最适反应温度为 5 0℃ ,最适pH为 4.8。用添加蜗牛酶的方法进行绿色木霉菌发酵 ,结果显示 ,分泌纤维素酶的能力提高 1 2 %左右。更多还原

【Abstract】 A strain was screened from the soil of Shanghai suburb, and was identified as Tichoderma virde . The experiment indicated that the optimum growth temperature of the strain was 28～30℃ and the optimum time of cellulase secreted was about 96 hrs. The hydrolytic level (the Filter Paper Activity, FPA) of cassava dregs was 10.8u/ml broth. The optimum conditions for cellulase action was at pH 4.8, 50℃. The fermentation yield of Trichoderma virde was increased by about 12% after adding snailase 500～800mg/L broth.更多还原