【摘要】 介绍了在原有数字化高灵敏度荧光显微镜的基础上研制的超高灵敏度荧光显微镜 ,对荧光实现 10 6数量级的增益 ,可获得细胞的光子灵敏显微图像 ,并结合图像融合技术 ,实现极微弱荧光图像的定位显示。采用具有视频速率的图像采集系统 ,可用于研究某区域荧光强度随时间变化的动力学过程。将该显微镜应用在生物医学领域的细胞凋亡的研究 ,观察到 As2 O3 诱导 MGC- 80 3细胞凋亡中 Ca2 + 浓度升高 ,预示着 Ca2 + 可能是由 As2 O3 启动 ,促发 MGC- 80 3细胞凋亡的又一重要机制。更多还原

【Abstract】 Based on the digital high sensitivity fluorescence microscope, the ultra high sensitivity fluorescence microscope is developed. The system can enhance the fluorescence intensity more than 10 6 and get the photon sensitive image of ultra weak fluorescence. The accurate position of ultra weak fluorescence can be confirmed by image fusion methods. Because the speed of image acquired system is 25 frame/s, the dynamic characters of fluorescence intensity can be investigated. Using the novel microscope to study the apoptosis of MGC 803 cells induced by As 2O 3, the density of Ca 2+ in apoptotic cells obviously increased. Ca 2+ is probably one of important roles in the process of MGC 803 apoptosis induced by As 2O 3.更多还原