【摘要】 为验证树对人乙型肝炎病毒 (HBV)的易感性 ,用含 HBV的人血清接种给 10只成年树 (雌雄各半 )。然后每周抽血 1次 ,每只动物共抽血 11次。用不同公司生产的 EL ISA试剂检测接种后的动物血清感染指标。实验观察至 13周 ,结果分别有 9只和 7只动物出现 HBs Ag阳性 ,持续最短 1周 ,最长 7周。用 PCR检测 ,结果有 1只动物连续 4周在血清中检测出 HBV DNA。表明树能感染人 HBV,但实验有待改进以延长感染持续时间更多还原

【Abstract】 To examine the tree shrew’s susceptibility for human hepatitis B virus,ten adult tree shrews (5 males,5 females) were inoculated by human sera with HBV. After that, weekly blood sampling was carried out one time each animal lasted for 11 weeks. HBsAg was detected with ELISA kits purchased from two different companies. Results showed that 9 and 7 animals in this experiment were positive for HBsAg respectively. One animal was found HBV DNA in sera by PCR, which lasted for 4 weeks. The data indicates that tree shrew could be infected with human HBV, but the methods of inoculation or conditions need to be improved to achieve a chronic HBV infection with more persistence of viral replication.更多还原