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内标-导数红外光谱法定量测定甲硫氨基酸的含量
Quantitative Determination of Methyl-sulf Amino-acid by Internal Standard-derivative Infrared Spetroscopy
【摘要】 本文利用内标 导数红外光谱法测定家禽营养限制性氨基酸———甲硫氨基酸的含量 ,选择了合适的内标物CaSO4,并采用峰高、峰面积、一阶导数、二阶导数等方法 ,所得的标准曲线方程的相关系数在0 994~ 0 998范围之间 ,结果比较满意。
【Abstract】 With pressed kBr disk technique the methyl sulf amino acid——the nutritious restrictive amino acid of poultry was determined by internal standard derivative infrared spectroscopy.The suitalbe internal standard——CaSO 4 was selected,and peak height,peak area,first derivative and second derivative methods were used.In the determination,we selected undisturbed peak at 553 cm -1 as standard.The correlation coefficient of standard curve equation were in the range of 0 994—0 998.The result was satisfied.
【关键词】 内标-导数红外光谱法;
甲硫氨基酸;
定量分析;
【Key words】 Internal standard-derivative infrared spectroscopy; Methy-sulf amino-acid; Quantitative determination;
【Key words】 Internal standard-derivative infrared spectroscopy; Methy-sulf amino-acid; Quantitative determination;
【基金】 内蒙古自治区教育厅和校优秀青年科研基金资助
- 【文献出处】 光谱学与光谱分析 ,SPECTROSCOPY AND SPECTRAL ANALYSIS , 编辑部邮箱 ,2000年04期
- 【分类号】O657.3
- 【被引频次】4
- 【下载频次】154