【摘要】 在红氏供试材料中苯丙氨酸转氨酶活性在分化愈伤组织中最高，但游离苯氨丙酸含量在分化愈伤组织中并无明显降低，说明游离氨基酸的积累主要来自蛋白质的分解。黄酮含量的变化以褐化愈伤组织中含量最高，因此我们推测：黄酮合成的最后几步，可能发生在褐化愈伤组织中，从可溶性蛋白质的ＳＤＳ－ＰＡＧＥ平板电泳结果表明，分化愈伤组织与褐化愈伤组织相比蛋白质谱带发生了明显的变化，这可能与控制黄酮合成的有关酶的活性变化有关。更多还原

【Abstract】 Activity of PAL is the hightest in differentiating callus of Hedysarum polybotrys Hand-Mazz. It is mot obvious that content of free PHE drop from callus to differentiating callus. It may be that free amino acids are accumulated with protein are divided. Content of flavone is the hightest in browning callus. It is supposed, that suppose which the last pace of synthesize flavone is in brovwing callus. SDS-PAGE cataphoresis electrophoresis show protein has big change from differentiating callus to browning callus. The change may has relation with change of enzymire activiation of control flavone synthesize.更多还原