【摘要】 通过发酵罐放大培养 ,优化发酵参数 ,提高了重组草鱼生长激素在酵母中的表达量。以含重组草鱼生长激素的酵母裂解物投喂鱼苗 ,实验组鱼体重的增加显著高于对照组 ,体长的亦高于对照组。鱼体生化组成分析表明 :投喂转草鱼生长激素基因酵母裂解物能显著提高鱼体的干物质和蛋白含量 ,并降低鱼体脂肪酸含量更多还原

【Abstract】 Recombinant grass carp GH was synthesized in methylotrophic yeast Pichia pastoris. High density cultivation of P.pastoris in a 5L fermentor was carried out to overexpress the recombinant growth carp GH using fed batch method. Expressed growth carp GH was to feed Yellow River carp (cyprinus carpio L.). The test groups grow faster and show significant enhancement in weight from 16 % to 53%. The content of dry matter and protein in the test groups is significantly higher and lipid is lower than that of control, respectively. The recombinant grass carp GH is potential to be used for the intensive fish culture.更多还原