【摘要】 采用 HRP逆行追踪结合谷氨酸免疫组织化学方法观察大鼠外侧膝状体背侧核 (d L GN)中继神经元的化学递质。光镜下 HRP标记细胞与谷氨酸免疫阳性细胞清晰可辩。HRP单标记细胞位于外侧膝状体背侧核内 ,胞浆及树突基部充满棕色颗粒。免疫金银法 (IGSS)单标记的谷氨酸免疫阳性神经元分布于外侧膝状体背侧核与腹侧核 ,胞体内充满黑色银颗粒。在外侧膝状体背侧核内可见 HRP和谷氨酸双标记细胞 ,其数目占 HRP标记细胞总数的 70 .9± 6 .4%。本文提示 ,谷氨酸可能是外侧膝状体背侧核投射至视皮质的中继神经元的神经递质之一。更多还原

【Abstract】 The retrograde HRP tracing technique combined with immunohistochemical method forglutamate was used to detect the neurotransmitters in relay cells which project to the visual cortex from dorsal lateral geniculate nucleus in rats.The HRP labelling neurons and glutamate immunoreactive cells were easily distinguished by the different reactive grains.HRP and glutamate double labelling neurons were found in the dorsal lateral geniculate nucleus accounting for70 .9± 6.4% ( X± Sn=1 5) in the total number of HRP labelling cells in the same area.The resultsuggests that glutamate is one of the neurotransmitters in the geniculo- cortical pathway.更多还原