【摘要】 体外进行器官培养 ,通过免疫组织化学和原位杂交方法检测香烟烟雾提取物 (CSE)作用 48、 72 h,小鼠气管上皮细胞上皮钙粘附素 (E- cd)表达的变化。结果表明 ,正常对照组 E- cd分布于气管假复层纤毛柱状上皮细胞连接处的胞膜上 ,5 0 % CSE作用后 ,膜上 E- cd表达减少 ,随着作用时间延长 ,胞浆内 E- cd表达明显增加 (P<0 .0 5 ) ,而细胞内 E- cd m RNA含量无明显变化。提示 ,CSE对小鼠气管上皮细胞 E- cd表达的调控在翻译后水平 ,胞膜上 E- cd表达的下调与吸烟所致气道上皮细胞的损伤修复过程相关。更多还原

【Abstract】 Mouse trachea was cultured in vitro, exposed to cigarette smoke extract (CSE), the change of E cadherin(E cd) expression on tracheal epithelial cells was determined by immunohistochemistry and in situ hybridization. The results showed that E cd was distributed on the membrane at the cell junctions of tracheal pseudostratified ciliated columnar epithelium. After exposure to 50% CSE, the membranous E cd expression decreased, the cytoplasmic E cd expression increased ( P <0 05) as the exposure time prolonged. But the content of E cd mRNA was not changed. It suggests that E cd expression is regulated at the posttranslational level, the downregulation of membranous E cd expression is related with injury and repair of tracheal epithelial cells caused by smoking.更多还原