【摘要】 目的　通过对Ⅰ型ＪＢＰ（ｃ 型） 和Ⅲ型６７１５（ｇ 型） 变链菌在不同状态葡糖基转移酶（ＧＴＦ） 所合成的水不溶性葡聚糖（ＩＧ） 和水溶性葡聚糖（ＳＧ） 的测定来研究两种变链菌的ＧＴＦ－Ｉ和ＧＴＦ－ Ｓ是否存在差异。方法　制备ＪＢＰ和６７１５ 的含酶上清液（ｃｅｌｌ－ｆｒｅｅ ｅｎｚｙｍｅ） 及含酶提取液（ｃｅｌｌ－ ｂｏｕｎｄ ｅｎｚｙｍｅ），以蔗糖为底物，恒温培养后分别收集ＩＧ 和ＳＧ。采用红外光谱、薄层层析及Ｓｃｏｔｔ 蒽酮定糖法对葡聚糖样品作定性、定量分析。结果　Ⅰ型ＪＢＰ（ｃ）含酶上清液主要合成ＳＧ，含酶提取液主要合成ＩＧ。Ⅲ型６７１５（ｇ）含酶上清液主要合成ＩＧ，含酶提取液合成ＩＧ稍多于ＳＧ，但无统计学显著性差异。对于含酶上清液ＩＧ 的合成，６７１５ 明显多于ＪＢＰ（Ｐ＜ ０ ．０５） ，而ＳＧ 的合成，ＪＢＰ明显多于６７１５（ Ｐ＜ ０．０５） 。含酶提取液ＩＧ的合成，ＪＢＰ明显多于６７１５（ Ｐ＜ ０．０５），而ＳＧ 的合成，６７１５明显多于ＪＢＰ（Ｐ＜ ０．０５）。结论　变链菌Ⅰ型ＪＢＰ的ＩＧ主要由细胞结合型ＧＴＦ 所合成，而变链菌Ⅲ型６７１５ 的ＩＧ主要由细胞游离型ＧＴＦ所合成。这种来自不同型ＧＴＦ 的ＩＧ 是否对变?更多还原

【Abstract】 Objective To study the differences of various types of GTF in Streptococcus mutans(serotype c) and Streptococcus sobrinus (serotype g) by determining water-insoluble glucans(IG) and water-soluble glucans(SG) synthesized from various glucosyltransferase. Methods The supernatant containing GTF and extraction containing GTF in these two streptococci have been prepared and glucans were synthesized. The thin layer chromatography and infrared spectra analysis were adopted to study the chemical nature and the chemical structure of glucans. Results IG presented predominantly in extraction of S.mutans and supernatant of S.sobrinus, while SG presented predominantly in supernatant of S.mutans. IG synthesized by the supernatant of S.sobrinus was more than that did by the supernatant of S.mutans, (P<0.05), whereas SG synthesized by the supernatant of S.mutans was more than that did by the supernatant of S.sobrinus (P<0.05), IG synthesized by the extraction of S.mutans was more than that did by the extraction of S.sobrinus(P<0.05). Conclusion IG was mainly synthesized by cell-bound GTF of S. mutans and SG was mainly synthesized by cell-free GTF of S.sobrinus. Further studies are required to prove the various types of GTF and whether their synthesizing IG affect streptococcus adhesion or not.更多还原