【摘要】 用激光闪光光解瞬态吸收光谱研究了水溶液中含芳香氨基酸残基肽的光敏化反应过程 .结果表明 ,在丙酮存在的含色氨酸残基肽 ( Trp-Gly,n-f-Met-Trp,Trp-Phe)体系的光解 ,丙酮三重态与 Trp分别通过三重态 -三重态 ( T-T)激发能转移和电子转移生成 Trp激发三重态和 N中心自由基 ( Trp/ N· ) ;丙酮三重态仅与含酪氨酸残基肽 ( Phe-Tyr)通过电子转移生成 Tyr酚氧自由基 ( Tyr/ O· ) .在色氨酰酪氨酸 ( Trp-Tyr)与丙酮的光解体系中 ,观察到分子内的电子转移 ,即由 Trp/ N· -Tyr→Trp-Tyr/ O·自由基的生成过程更多还原

【Abstract】 The photochemical processes of triplet acetone with aromatic amino acid containing peptides were investigated in neutral aqueous solution using KrF(248 nm) laser flash photolysis. In the presence of acetone, the formation of excited triplet states of tryptophan residues and radicals(Trp/N ·) via a triplet triplet( T T ) energy transfer and an electron transfer respectively, was observed in case of Trp Phe, Trp Gly and n formyl Met Trp, while only phenolic hydroxy radical(Tyr/O ·), via an electron transfer, was observed in case of Phe Tyr. Furthermore, triplet acetone induced radical transformation: Trp/N · Tyr→Trp Tyr/O · was observed directly, and the transformation resulting from intramolecular electron transfer was suggested. [WT5HZ]更多还原