【摘要】 以球形纤维素为载体 ,经环氧氯丙烷活化后共价键联小牛胸腺 DNA,制备 DNA免疫吸附剂 ,通过血液灌流能够治疗系统性红斑狼疮 .对病人血清的吸附实验结果表明 ,每毫升吸附剂与 3 m L病人血清混合 ,于 3 7℃保温 1 h,可吸附除去 4 0 %～ 70 %致病抗体更多还原

【Abstract】 In order to remove the anti DNA antibody from the blood of patients with systematic lupus erythematosus（SLE）, DNA immunoadsorbent was prepared by coupling calf thymus DNA to epichlorohydrin activated cellulose beads. The methods and conditions for activation of carrier beads, DNA coupling and adsorption with patient’s plasma were investigated. The activation level was found to be dependent on the NaOH concentration, amount of offered epichlorohydrin and the molar ratio between NaOH and epichlorohydrin. One milliliter of cellulose beads was activated by incubation with 0.5 mL of epichlorohydrin and 2.0 mL of 2.0 ? ?.0 mol/L NaOH solution at 40 ℃ for 2 h, and the activation level was 60₁00 μmol/mL beads. DNA coupling was found to be strongly dependent on temperature and pH. The optimal coupling conditions were 0.1 mol/L Tris HCl buffer, pH 7.9 ? ?.0 and 70—90 ℃. High activation level, high DNA concentration and long reaction time were also required. Under the optimal condition, 2.0 ? ?.0 mg of DNA could be coupled to 1.0 mL of cellulose beads. In vitro adsorption tests showed that incubation of 1.0 mL of adsorbent with 3.0 mL of plasma could remove 40%—70% of anti DNA antibody from the plasma. [WT5HZ]更多还原