【摘要】 考察了用微乳液毛细管电动色谱 ( MEEKC)分离蛋白质时微乳液组成等不同因素对分离的影响 ,并与胶束电动色谱进行对比 ,探讨了其分离机理 ,为蛋白质的分离鉴定提供了一种有力的工具更多还原

【Abstract】 Microemulsion electrokinetic chromatography(MEEKC) is a new technique which was employed to separate both basic and acidic proteins simultaneously. Optimum separation conditions were investigated with respect to ionic strength and the composition of microemulsion. The results show that if a stable microemulsion formed, the efficiency of protein separation was slightly affected by the concentration of the background and the composition of microemulsion. The fundamental characteristics of MEEKC were studied in comparison with micellar electrokinetic chromatography(MEKC). The mechanism of MEEKC was also investigated. The results show that MEEKC is an alternative way for protein separations with a high efficiency.更多还原