节点文献
以人血清白蛋白为固定相的分子生物色谱分析几种中药活性成分的研究
Analysis of Biologically Active Compounds in Chinese Medicines by Molecular Biochromatography with Human Serum Albumin(HSA) Stationary Phase
【摘要】 提出用人血清白蛋白分子生物色谱分析中药活性成分的方法 ,分别比较了当归等 4种单味中药的分离结果 ,考察了提取当归的 3种溶剂及提取时间对活性物质提取量的影响 ,同时建立了当归中阿魏酸与藁本内酯的定量分析方法 .
【Abstract】 A strategy for analysis of bioactive compounds in traditional Chinese medicine was first proposed by molecular biochromatography with human serum albumin(HSA) stationary phase. Separation of active compounds in four kinds of traditional Chinese medicine(TCM) was compared on the HSA column, and found that different TCM show different characteristic of chromatogram patterns. The effects of extracting solutions(water/methanol/ethanol) and extracted time on the amount of bioactive compounds in Angelica sinensis(Oliv.) Diels were examined, and a quality control method was also developed based on this technique, which is able to simultaneously determine the concentrations of ferulic acid and liguistilide in extracted solutions of Angelica sinensis(Oliv.) Diels by water and methanol.
【Key words】 Human serum albumin; Molecular biochromatography; Traditional Chinese medicine; Angelica sinensis(Oliv.) Diels; Bioactive compound;
- 【文献出处】 高等学校化学学报 ,CHEMICAL RESEARCH IN CHINESE UNIVERSITIES , 编辑部邮箱 ,2000年01期
- 【分类号】O657.7
- 【被引频次】71
- 【下载频次】850