【摘要】 将 2 -氨乙基硫醇 (AET)固载到玻碳电极 (GCE)表面 ,进而化学吸附纳米金 (NG) ,并在纳米金上固载ss-DNA得到 ss-DNA/NG/AET/GCE,以 Co(bpy) 3+ 3为电化学指示剂可以识别研究 ss-DNA的杂化反应 .结果表明 ,纳米金能使固载其上的 ss-DNA发生部分变性而结合 Co(bpy) 3+ 3,但用 p H7.0的磷酸缓冲液浸泡可基本上避免这种变性 ,并明显提高杂化反应的识别能力 .结合在 ds-DNA/NG/AET/GCE上的 Co(bpy) 3+ 3的峰电流与扫速的线性关系可保持到 80 m V/s.与电沉积法固载纳米金相比较 ,本电极更稳定和可靠 .更多还原

【Abstract】 The modification of glassy carbon electrode（GCE） with nano-gold（NG） by chemical adsorption of 2-aminoethane thoil（AET） was achieved for the first time. On this electrode 15-mer ss-DNA was immobilized, then ss-DNA/NG/AET/GCE was formed. By using Co（bpy）3+₃, hybridization events of the immobilized ss-DNA was well detected. It was found that the NG could possibly cause a part of the ss-DNA to denature and bind Co（bpy）3+₃. However, soaking in pH 7.0 phosphate buffer solution, denaturization can be avoided and recognition ability of hybridization was enhanced. Cyclic voltammetric behavior of Co（bpy）3+/2+₃ bound to the ds-DNA/NG/AET/GCE was studied. It was found that the peak current（i_p） showed a linear dependence on the potential scan rate up to 80 mV/s. It was demonstrated that GCE modified with NG by this method had higher stability and reliability.更多还原