【摘要】 目的　了解荧光定量聚合酶链反应 (PCR)法与分支链DNA信号扩增 (bDNA)法在检测血清中HBVDNA含量上的优缺点。方法　分别用这两种方法平行检测 44份乙型肝炎患者的血清标本 ,并与定性PCR检测结果比较。同时用荧光定量PCR法观察 15例乙肝患者干扰素治疗期间HBVDNA含量变化。结果　①荧光定量PCR法和bDNA法比较 ,○a前者测出的HBVDNA含量均值为 ( 3 5 0× 10 5± 3 3 6)copies/μl ;后者为 ( 3 0 7× 10 5± 12 9)copies/μl ,两者比较差异无显著性 (P >0 0 5 )。○b前者的HBVDNA阳性率 79 5 % ;后者及常规PCR定性法均为 5 9 1%。荧光定量PCR法的阳性检出率显著高于bDNA法及常规定性PCR法 (P <0 0 5 )。② 15例中 5例获完全应答的乙型肝炎患者其血清HBVDNA含量在治疗 16周内均逐渐下降至完全转阴。结论　荧光定量PCR法与bDNA法在检测血清HBVDNA含量上 ,结果大多一致 ,且敏感性强 ,检验也较简便、价廉 ,并能较好评价和检测乙肝患者抗病毒疗效 ,宜于临床推广。更多还原

【Abstract】 Objective To compare the amplisensor quantitative PCR assay with the branched-DNA signal-amplification assay in detecting HBV DNA in clinic.Methods Sera from 44 hepatitis B patients were measured by the two assays and also by qualitative PCR assay. Data from these three assays were compared. In addition, the changes of HBV DNA quantitative value in 15 hepatitis B patients with Interferon therapy were studied.Results ①The data of HBV DNA quantitative value measured by these two methods had no significant difference.②The HBV DNA positive rate of the amplisensor quantitative PCR assay is much higher than that of both the bDNA assay and the HBV DNA qualitative assay.③ The HBV DNA quantitative value in 5 patients (5/15) declined gradually and became negative within 16 weeks of interferon therapy. Conclusions Compared with the bDNA assay, the amplisensor quantitative PCR assay is more sensitive, reliable, simpler and cheaper, and is therefore more useful to evaluate the effect of anti-viral therapy.更多还原