【摘要】 目的观察谷氨酰胺（ＧＬＮ）对急性坏死性胰腺炎（ＡＮＰ）大鼠肠道细菌移位的影响。方法将实验大鼠１２０只，随机分成对照组、ＡＮＰ组及ＧＬＮ组，每组各４０只，ＡＮＰ模型采用结扎胰胆管诱发，ＧＬＮ组制作ＡＮＰ后灌入ＧＬＮ，观察３组肠粘膜组织学和 ＤＮＡ含量，肠系膜淋巴结，胰腺组织细菌培养及吖啶橙标记菌实验。结果 ＡＮＰ后 ７２ ｈ，肠粘膜出现明显损伤， ＤＮＡ含量下降，肠系膜淋巴结，胰腺组织细菌培养计数明显升高，吖啶橙标记菌移位率明显升高，应用 ＧＬＮ能明显减轻肠粘膜损伤，降低上述指标。结论ＧＬＮ有利于维持ＡＮＰ时肠粘膜屏障功能，减少肠道细菌移位的发生。更多还原

【Abstract】 Objctive To study the influence of Glutamine on bacterial translocation of rats with acute necrotizing Pancreatitis (ANP). Method 120 rats were divided randomly into three groups (control, ANP and Glutamine group)(n =40, each). ABP model was induced by Obstruction of biliopancreatic duct. The rats in Glutamine group were fed with glutamine after ANP induction. The histology and DNA content of intestinal mucosa, the number of bacteria cultured and E. coli labelled with acridine orange in mesentery lymphy node(MLN) and pancrease were observed. Results There were obvious lesions in the intesinal mucosa DNA centent in mucosa significantly decreased (P < 0.01). The number of bacteria culted and E, Coli labelled with acridine orange in MLN and pancresseincreased significantly (P < 0.01) in ANP group. Mucous injury and the number of bacteria could be reduced by Glutamine. Conclusion Glutmine may help to maintain the mucosal barrier function and reduces bacterial translocation in ANP.更多还原