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鸡卵黄免疫球蛋白的嗜硫性色谱一步分离纯化
One-step thiophilic adsorption chromatography purification of chicken immunoglobulin from egg yolk
【摘要】 采用重力沉降法对卵黄液进行脱脂预处理 ,利用嗜硫性色谱 ,实现鸡卵黄免疫球蛋白(IgY)的一步分离纯化 ,蛋白质的回收率高达 91% .鸡卵黄免疫球蛋白IgY经SDS -PAGE检测为电泳纯 ,活性回收率达 70 %以上
【Abstract】 This paper described the separation and purification of chicken IgY by using thiophilic adsorption chromatography (TAC). The lipoprotein was removed by gravity settling followed by filtration. The IgY with TAC was found to be homogeneous by SDS-PAGE. The biological activity of IgY and the recovery of total protein were more than 70 % and 91 %, respectively.
【关键词】 卵黄抗体;
IgY;
沉降;
嗜硫色谱;
【Key words】 chicken immunoglobulin; IgY; gravity settling; thiophilic adsorption chromatography;
【Key words】 chicken immunoglobulin; IgY; gravity settling; thiophilic adsorption chromatography;
【基金】 福建省计委攻关项目!(QG98- 4 )
- 【文献出处】 福州大学学报(自然科学版) ,Journal of Fuzhou University(Natural Sciences Edtion) , 编辑部邮箱 ,2000年06期
- 【分类号】TQ93
- 【被引频次】25
- 【下载频次】208