【摘要】 报道了一步洗脱离子交换色谱法分离纯化蛋黄水溶性组分 (WSF)中IgY的新方法 .通过将含IgY的WSF用pH7.0磷酸盐缓冲液 (PBS)调整至盐浓度为 0 .0 75mol L ,上样到DEAEToyoprarl6 50M阴离子交换色谱柱 ,用 0 .15mol LpH7.0PBS洗脱、收集 ,再经SDS -聚丙烯酰胺凝胶电泳检验为单一组分 .分离方法简单、分离纯化效果好 ,总蛋白回收率达 93%以上更多还原

【Abstract】 This paper reported an improved method for separation and purification of IgY from egg water-soluble fraction (WSF) by one step elution anion-exchange chromatography. The prepared WSF was adjusted to contain 0.075 mol/L sodium phosphate buffer, pH7.0 before being loaded to DEAE Toyopearl 650M column. IgY was eluted with 0.15 mol/L sodium phosphate buffer, pH7.0, and the fraction was tested to be electrophoretically pure, with protein recovery of 93%. Proved to be highly efficient with high recovery, this novel method can be applied in large scale preparation of IgY.更多还原