【摘要】 为了进一步探讨胰岛淀粉样多肽（ＩＡＰＰ）的分布、定位以及它与其他生物活性物质的关系；用ＩＡＰＰ组织化学ＰＡＰ邻片双标法，观察了１８例１４～３８周人胎气管内ＩＡＰＰ免疫反应（ＩＲ）细胞的个体发生及与５羟色胺（５－ＨＴ）的关系。结果显示，胎１４周，气管粘膜表面的假复层柱状上皮中已有ＩＡＰＰ－ＩＲ细胞（Ｆｉｇ．１＆２）；１５周开始，粘膜固有层气管腺导管上皮中也出现分散的ＩＡＰＰ－ＩＲ细胞（Ｆｉｇ．３）；随胎龄增长，１７～２１周，气管上皮内ＩＡＰＰ－ＩＲ细胞逐渐增多；免疫染色加深（Ｆｉｇ．４＆５），有些细胞发出细突直达腔面（Ｆｉｇ．６＆７），粘膜下层的气管腺腺泡中也有ＩＡＰＰ－ＩＲ（Ｆｉｇ．８）； ２２～３８周，气管内 ＩＡＰＰ－ＩＲ 细胞又呈逐渐减少趋势，ＩＡＰＰ－ＩＲ仅出现在基底锥形细胞中（Ｆｉｇ．９＆１０），且免疫染色较深。邻片未显５－ＨＴ－ＩＲ。本研究表明，人胎儿期气管上皮细胞内有ＩＡＰＰ的表达；且ＩＡＰＰ－ＩＲ细胞随胎期的发育而发生变化。更多还原

【Abstract】 In order to further study on the localization, distribution of islet amyloid polypeptide (IAPP) and the relationship between IAPP and other bioactive substances, the ontogeny of IAPP immunoreactive (IR) cells and the relationship between IAPP and 5-hydroxytryptame (5-HT) in human fetal tracheas from 18 fectuses of 14- to 38-week age of gestation were observed with double labeling immunocytochemical staining technique on adjacent section. The results showed that at the 14th week, there existed the IAPP-IR cells in the pseudostratified ciliated columnar epithelium of fetal trachea. The IAPP-IR cells scattered also in the glandular duct epithelium of human fetal trachea from the 15th week of gestation. During the 17th-21st weeks of gestationa, the number of IAPP-IR cells gradually increased and the immunostaining of cells turned more intensive along with age. From the 22nd to 38th week, the number of IAPP-IR cells decreased gradually and IAPP-IR cells appeared in the basal cone-shaped cells with the lighter immunostaining. The adjacent sections were not shown 5-HT immunoreactivity. The results indicated that the IAPP was expressed in the trachea epithelium of human fetus and the IAPP-IR cells varied along with the development of fetus.更多还原