【摘要】 观察了电离辐射对金属硫蛋白（ＭＴ）在免疫器官中表达的影响。采用放射性１０９Ｃｄ血红蛋白亲和分析法检测组织中 ＭＴ含量。观察 ０．５、 ２、 ４、 ６Ｇｙ Ｘ射线全身照射后 １６ｈ，及４Ｇｙ全身照射后 ０．５、 ２、 ４、 ８、 １６、 ２４、 ４８、 ７２ｈ，昆明小鼠胸腺及脾脏中 ＭＴ含量的变化。结果发现，在 ０．５～６Ｇｙ剂量范围内胸腺中 ＭＴ含量呈剂量依赖性增高，其中， ４Ｇｙ和 ６Ｇｙ组有统计学意义（ｐ＜０．０５）。 ４Ｇｙ Ｘ射线照射后 ８～４８ｈ胸腺中 ＭＴ含量显著增高（ｐ＜ ０．０５～ｐ＜０．００１）。然而，量效及时效研究均未见脾脏中 ＭＴ 含量的变化．以上结果表明，Ｘ射线能诱导ＭＴ在免疫器官中表达，但表现出明显的组织特异性。更多还原

【Abstract】 To explore the effect of ionizing radiation on the expression of metallothionein (MT) in immune organs, the 109 Cd-hamoglobulin affinity assay was employed to measure the content of MT in tissues. The dose effect relationship of the MT expression in mouse thymus and spleen was investigated 16h after exposure to 0.5, 1, 2, 4 and 6Gy whole body irradiation (WBI). And the time course changes in MT expression in mouse thymus and spleen were also determined at different time intervals after 4Gy WBI. The results showed that the MT content in mouse thymus was significantly increased 16h after 4Gy and 6Gy irradiation compared with that in sham-irradiated mice (p <0.05, respectively). And it was found that the MT content in mouse thymus was also increased significantly from 8h to 48h after 4Gy irradiation compared with that in sham-irradiated mice (p < 0.05 - P <0.001). However, there were no markedly changes for the MT expression in the mouse spleen after exposure to X rays. These results mentioned above suggest that the MT expression in immune organs could be induced by X rays, which may be tissue-specific.更多还原