【摘要】 结合生命科学中生物活性物质的高灵敏度分析检测方法研究 ,组装了时间分辨激光荧光免疫分析装置 ;合成了稀土离子与蛋白质联接的双向螯合剂 二乙三胺五乙环酐(DTPAA) ;纯化了抗 乙型肝炎表面抗体 ;研究了Eu3+ DTPAA 抗 乙型肝炎表面抗体制备过程 ;确定了标记物制备的最佳条件 ;依据解离增强原理 ,采用时间分辨激光荧光光谱技术 ,对Eu( β NTA) 3(TOPO) 2 螯合物的有关参数进行了测定。更多还原

【Abstract】 A microsecond time resolved laser fluorescence spectroscopic analysis set was developed. A chelate cyclic anhydride of diethylenetrimin pentaacetic acid anhydride (DTPAA) was synthesized. An anti HBs antibody was purified. A Eu 3+ DTPAA anti HBs label was prepared by two step procedure. We described the optimal condtion with Eu 3+ as marker and DTPAA as chelate bounding to antibody molecule. Labeling parameters such as solvent pH, protein and chelate molar ratio, reaction time, separation method were discussed in detail.更多还原