【摘要】 采用微机械加工技术制作了具有三维结构的微腔型传感器芯片（腔深３００μｍ，铂工 作电极１．２×１．２ｍｍ２）。作者对微腔型电极进行了电化学特性的表征。并通过明胶包埋法将 半乳糖氧化酶固定在电极中，构成微腔型半乳糖传感器。该酶电极对半乳糖测定的上限为 ４．５ｍｍｏｌ／Ｌ，相关系数为０．９９８０。较适宜的ｐＨ范围为７．０～７，５，响应时间＜５０ ｓ。该酶电极 在１４天内连续使用，响应电流无明显变化。放置一个月后测试仍保持较好的电流响应。更多还原

【Abstract】 A microchip with three dimensional silicon chamber (l. 2 x 1 .2 mm2 and 300 μm thick) was developed for the construction of micro-size biosensor. The electrochemical characterization of its Pt working electrode was reported. A galactose biosensor was constructed with the substrate working electrode of chamber-type microchip. The upper detection limit of the microsensor is 4. 5 mmol/L and the response time was less than 50 s. No significant variation was observed in detection within two weeks.更多还原