【摘要】 采用核磁共振弛豫分析法研究了造影剂母体化合物钆-二乙三胺五乙酸（Gd-DTPA）在水溶液和牛血清白蛋白溶液中的诱导弛像增强性质。Gd-DTPA络合物在水溶液中弛豫效率为5．25mmol-1·L·s-1，并发现牛血清白蛋白分子可非共价地结合Gd－DTPA络合物，这种非共价结合体的旋转相关时间明显长于自由络合物，使Gd-DTPA在蛋白质溶液中弛豫效率增高。本文结果表明该方法可以用来研究顺磁性金属络合物与蛋白质之间的相互作用情况。更多还原

【Abstract】 In this paper, the water relaxation enhancemen behavior of Gd-diethylenetriamine pentaacetic acid(DTPA) in water and in aqueous solution of bovine serum albumine (BSA) has been studied. The T1relaxivity of Gd-DTPA in BSA solution is higher than that in aqueous solution. The results indicate that Gd-DTPA can integrate non-covalently with BSA mainly in forms of (Gd-DTPA)·BSA、 (Gd-DTPA)2·BSA, for which the apparent equilibrium constant is 0.026 mmol-1·L、0.0018 mmol-2·L2 respectively.This method would be used to study the interactivities between protein and contrast agent.更多还原