【摘要】 研究了银杏叶提取物中5种银杏内酯含量的测定方法。外标法定量，反相Symmetry-C18柱，流动相为甲醇-水；示差折光检测器。线形范围为0．02~0．40g/L，r＝0．9814~0．9934，方法的回收率和相对标准偏差分别在93．2％~97．4％和1．27％~2.68％（n=15）之间，5种银杏内酯的检测限在2．07~3．98mg/L之间。本法简便、准确、重现性好，适宜于生产和工艺研究的质量控制。更多还原

【Abstract】 In this paper, five kinds of gingkolides(GA,GB,GC,GJ, BB) in gingko biloba leaves extract was determined by reversed-phase high performance liquid chromatography at the first time. The Waters Symmetry-C18 colurnn(3. 9 mm× 150 mm, 5 μm) and column temperature was 30℃, 24% (V/V) methanol as mobile phase and detector was refractometer (model: waters410, sensitivity: 1024). The calibration curve was linear in the range of 0.02 ~ 0.40 g/L, r = 0.9814 ~ 0.9934; recovery: 93 .2% ~97.4% and RSD: 1 .27% ~ 2.68%,different ginkgolides determination limit from 2.07 to 3.98 mg/L.This method is sensitive, reproducible and easy to operate.The condition of column can be enlarging for preparinge-scale high performance liquid chromatography.更多还原