【摘要】 应用免疫组织化学染色 ,观察人脑挫伤后 S- 10 0和 GFAP时间变化的规律 ,为脑挫伤形成时间的推断提供新的形态学依据。S- 10 0阳性细胞面积、灰度在伤后 48h显著增加 (P<0 .0 1) ;伤后 4d阳性细胞面积、灰度达到峰值 (P<0 .0 1) ;伤后 11d仍然保持较高水平 (P<0 .0 1)。GFAP阳性细胞面积、灰度伤后 2 4h显著增加 (P<0 .0 1) ;7d达高峰 (P<0 .0 1) ;以后有逐渐下降的趋势。伤后人脑组织 S- 10 0和 GFAP免疫组织化学染色阳性细胞面积、灰度改变有时间规律 ,两者可作为在 2～ 2 0 d内不同时间脑损伤的推断指标更多还原

【Abstract】 For the purpose of studying changes of S-100 and GFAP of nerve cells in human brain after brain contusion,38 cases were studied by the immunnohistochemistry combined with quantitative image analysis.The results showed that the grey degree and the area of S-100 positive cells increased 48 hours after brain injury and reached to the maximum on the 4th day.Since then it decreased.GFAP positive cells increased significantly 12 hours after brain contusion and reached to the maximum on the 4th day and kept at the high level until the 7th day.The results suggest that the changes of S-100 and GFAP during the different intervals were regular.So that,both S-100 and GFAP can te used as markers of brain contusion in the interval of 2～20 days after brain contusion更多还原