【摘要】 目的 研究Ki67表达和HPV感染在宫颈癌发生发展过程中的意义。方法 分别从正常宫颈(10例)、各级CIN(CIN Ⅰ19例,CIN Ⅱ9例,CIN Ⅲ16例)和宫颈鳞癌(8例)的石蜡标本提取基因组DNA,选取HPV L1区通用引物进行PCR扩增,测序,与已知HPV序列进行同源性分析。Ki67免疫组化染色。结果 正常宫颈组HPV DNA均为阴性。CIN Ⅰ组5/19例为高危型HPV(16/18型),8/19例为中危型(35型等),其余为低危型。CINⅡ和Ⅲ组高危和中危型HPV各占一半。宫颈癌组均为高危型,绝大部分为HPV16。Ki67指数随CIN级别的升高(CINⅠ:21.4±1.1,CINⅡ:31.8±3.5 CINⅢ:61.3±2.8)而明显增加(P<0.01)。结论Ki67指数反映出在宫颈上皮细胞癌变过程中细胞增殖活性的改变。HPV型别与CIN级别及转归密切相关。Ki67与HPV检查联合应用对评价CIN细胞增殖活性及其转归有重要的作用,对伴有HPV16/18 感染的CIN应密切追踪和积极处理。更多还原

【Abstract】 Objective To study the expression of Ki67 antigen and human papillomavirus (HPV) infection in cervical intraepithelial neoplasia (CIN) and cervical carcinoma. Methods Ki67 antigen immunostaining and HPV typing were investigated in 10 normal cervix, 19 CIN I , 8 CIN II , 16 CIN III and 8 cervical squamous carcinoma. Results HPV DNA was not detected in normal cervix. HPV 16/18 were detected in 5 of 19 CIN I..One half of CIN II and III were high-risk HPV positive, the others were intermediate - risk HPV positive. Nine out of 10 cervical carcinoma contained HPV16, only one contained HPV 18.The Ki67 index significantly increased with the increasing grade of CIN. Conclusions Both Ki67 immunostaining and HPV typing illustrate important clinical values to investigate the cell proliferation and prognosis of CIN.更多还原