【摘要】 目的　研究弓形虫与宿主细胞之间相互作用的钙通道机制。　方法　以急性期感染小鼠腹水收集的RH株刚地弓形虫速殖子为材料 ,采用荧光染料 Fura- 2与细胞质游离钙结合 ,通过荧光分光光度计技术和 SuperIon Probe Software计算机软件测定弓形虫速殖子细胞质游离钙浓度 ([Ca2 + ]i)。　结果　正常弓形虫速殖子[Ca2 + ]i为 (2 0 5 .0 2± 18.6 ) nm ol/ L,符合文献报道的静息状态下真核细胞 [Ca2 + ]i浓度范围。　结论　本方法简便、易行 ,相对费用低 ,适用于国内实验室更多还原

【Abstract】 Objective\ To further study the mechanism of Ca\+\{2+\} channels in interaction between both toxoplasma and host cell.\ Methods\ Toxoplasma gondii tachyzoites of the RH strain collected from the peritoneal fluid in mice 3 days after infection,using a fluorescence dye(Fura\|2) to bind cytoplasmic free Ca\+\{2+\}.\ Cytoplasmic free calcium concentration([Ca\+\{2+\}]i) in toxoplasma gondii tachyzoite was determined by spectrofluorophotometer and Super Ion Probe Software computer software.\ Results\ The cytoplasmic free[Ca\+\{2+\}]i in nomal toxoplasma gondii tachyzoite were(205.02±18.6)nmol/L, concentration which are in the range observed in many studies with resting eukaryotic cells.\ Conclusion\ This method for measurement of Ca\+\{2+\} is simple, applicable and less expensive.\;更多还原