【摘要】 采用水作为溶剂模拟尿液中药物并对其进行定量分析 ,探讨了荧光分析法对人体内药物代谢程度的研究。阐述了荧光猝灭剂、敏化剂及浓度效应对其的干扰 ,描述了荧光分析法药物定量分析的规程。从 1×10 -7g/mL血噗啉水溶液的荧光光谱实验中观察到含Fe 化合物浓度在 10 -4 g/mL足以产生严重影响 ,引起荧光猝灭 ;图 1、图 2分别示出了浓度为 1× 10 -3g/mL和 1× 10 -4 g/mL的盐酸阿霉素的荧光光谱和激发光谱。比较可以看到低于 1个数量级的盐酸阿霉素的发光强度却高。浓度猝灭现象可以从光谱的移动来判断 ,随着样品浓度的降低 ,发光强度却增强 ,光谱发生蓝移或红移。排除浓度效应干扰的方法是增加不同浓度发光强度的测量点 ,找出线形区域后 ,应用实验结果和 (1)式来分析 ;采用Fo¨rster理论讨论了能量施、受主之间的敏化机制。实验给出了噗啉、盐酸阿霉素、丝裂霉素、依托泊甙、硫酸醛长春碱的荧光光谱和激发光谱 ;检测限分别达到 10 -3 ～ 10 -7g/mL ;表明其完全可以满足此类药物对检测灵敏度的要求更多还原

【Abstract】 The metabolic level of medications which treat cancer was studied by fluorescence spectroscopic analysis in the patients body, the medications were quantitatively analyzed in the urine that was simulated with water as solvent. The measuring conditions were discussed, including the effects of fluorescence quenching, sensitization medium and the medication concentration change. The method of quantitative analysis was researched for the medication metabolizing in body by fluorescence spectroscopy, and the sensitizing pattern between donor and acceptor of energy was discussed with F o ¨rster theory in this paper. The results show that fluorescence is quenched as the compound containing Fe  concentration is 1×10 -4 g/mL in 1×10 -7 g/mL HPD (hematoporphyrin); the luminescence intensity of poxorubicin hydrochloride is higher as its concentration is lower from contrasting concentration 1×10 -3 g/mL to 1×10 -4 g/mL for fluorescence and excitation spectra, respectively, and the spectra are shifting red or blue when the concentration quenching arise, then can get rid of the interference of concentration effect by changing the point of measuring luminescence intensity on the line area, and the fluorescence and excitation spectra of Poxorubicin Hydrochloride; Etoposide; Vincristinal Sulfas and Mitomycin c for injection could also be obtained from this experiment. The detection limit of this method can achieve 10 -3 ～10 -7 g/mL, and this results show that detection sensitivity of fluorescence spectroscopy is higher than those of other analytic methods.更多还原