【摘要】 一条长 3 44 6bp的人类新基因cDNA已从胎脑cDNA文库中被克隆 .该cDNA克隆包含长 2 5 83bp的开放读框 ,推测编码一条长 86 0个氨基酸残基的蛋白质 ,预测分子质量为 96 .8ku .通过同源性比较及profile搜索 ,预测氨基酸序列显示出DExH BOXRNA解旋酶基因家族特有的 7个保守基序及已知RNA解旋酶氨基酸序列的较高同源性 ,进一步通过建立蛋白质结构模型同已知RNA解旋酶结构的比较 ,可以认定其为一条人类RNA解旋酶基因家族新成员 .通过该基因编码的氨基酸序列中基序ⅡD E V H残基将其命名为DVH(DEVH boxHelicase) .生物信息学手段结合DVH基因表达谱分析结果可推定DVH可能在胎儿发育过程中起作用 .由于解旋酶基因家族同人类遗传疾病关系密切 ,以上结果可指导DVH基因的进一步功能研究并提示该基因作为疾病侯选基因的可能性更多还原

【Abstract】 A cDNA clone encoding a putative RNA helicase has been isolated from a human fetal brain cDNA library. The cDNA sequence is 3 446 bases long, and its largest open reaing frame encodes 860 amino acids. The central region of the predicted protein, which is homologous to a group of nucleic acid helicases, fits well the 7 conserved motifs of DExH box RNA helicase family. Depending on the results of alignment and 3D modelling by 3D PSSM, the predict protein is regarded as a putative new member of RNA helicase family, and designated as DVH (DEVH box Helicase). Homology comparing shows that DVH may be a developmental processes related protein and this hypothesis is reinforced by expression profile analysis on cDNA microarray data.更多还原