【摘要】 目的　探讨FHIT基因转录表达异常在人肺癌发生、发展中的作用。方法　应用nestedRT PCR方法对 3 5例人非小细胞肺癌 (NSCLC)组织、癌旁组织和远癌肺组织 ,10例肺良性病变肺组织以及 4株肺癌细胞株中FHIT基因转录表达异常进行了研究。结果　 3 5例肺癌中 14例肺癌组织存在FHIT转录表达异常 ,异常率为 40 % ;在这 14例癌组织转录本异常病例中 ,5例癌旁组织亦见转录本异常( 3 5 .71% ,5 /14 )。远癌肺组织和肺良性病变组织均未见转录本异常。 4株肺癌细胞株均检测到转录本异常。在鳞癌中FHIT基因转录本异常率 ( 5 8.82 % )显著高于腺癌 ( 2 2 .2 2 % ) (P <0 .0 5 )。FHIT基因转录本异常与肺癌细胞的分化程度、P TNM分期、原发肿瘤大小、肿瘤部位 ,以及患者年龄等均无明显关系 (P>0 .0 5 )。结论　本研究结果表明在NSCLC中存在FHIT基因转录表达异常。FHIT基因异常主要发生在肺鳞癌中 ,且可能是肺癌发生的早期分子事件。更多还原

【Abstract】 Objective To explore the role of abnormalities in RNA transcript of FHIT gene in human non small cell lung cancer(NSCLC). Methods Transcript abnormalities of FHIT gene were detected in 35 cancer samples and their corresponding paracancer tissues and normal tissues, four lung cancer cell lines, and 10 lung tissues of benign pulmonary lesions as control by nested RT PCR. Results Fourteen of 35 cancer tissues (40%) had abnormalities in RNA transcripts of FHIT. There were 5 paracancer tissues with transcript abnormalities of FHIT gene out of the 14 lung cancers with transcript abnormalities of FHIT gene, with an abnormality rate of 35.71%. All of 4 lung cancer cell lines had transcript abnormalities of FHIT gene. The aberrant rate of FHIT gene in squamous cell carcinoma tissues (58.82%) was significantly higher than that in adenocarcinoma tissues (22.22%) (P<0.05). No relationship was found among the transcript abnormality of the FHIT gene and differentiated degree of the tumor cell, stages of the cancer, size of the primary tumor, location of cancer, and age of the patients (P>0.05). Conclusion The results show that there are transcript abnormalities of FHIT gene in NSCLC. FHIT transcript abnormality preferentially occur in squamous cell carcinomas, and it might be the early molecular phenomenon of lung cancer.更多还原